Join us in celebrating our 50th Anniversary!
Join us in celebrating our 50th Anniversary!

M54: Principles and Procedures for Detection and Reporting of Fungi in Clinical Specimens—Microscopic Examination, Antigen Detection, and Culture, 2nd Edition

CLSI requests nominations for volunteers to serve as members and contributors on a document development committee for a document revision.


CLSI document M54 will provide protocols, quality control parameters, and breakpoints for collecting, processing, handling, detecting, and identifying fungi in direct specimen examinations. This revision will include updated fungal taxonomy, along with direct molecular methods for detecting Candida spp. from blood by magnetic resonance, Pneumocystis jirovecii from respiratory specimens by polymerase chain reaction, and the emerging pathogen Candida auris. This guideline will consider individualized quality control plan issues related to fungal media and include a table listing the differential diagnoses of various yeasts on direct examination. These recommendations will apply to laboratories worldwide and provide the impetus for international consensus.

This group is looking for volunteers with:

  • Experience in and knowledge about the design and development of fungal culture tests for clinical and industrial applications
  • Experience in and knowledge about the validation and verification of fungal culture tests for clinical and industrial applications
  • Technical expertise in the culture and/or molecular detection of infectious diseases
  • Experience in and knowledge about the interpretation and reporting of fungal culture test results

Additional information on essential job duties and skills and abilities for CLSI committee participants can be viewed here.

Timeline for this project

January 24-25, 2019: First face-to-face meeting (NOTE: This will be a 2-day meeting scheduled during the January Committees Week in St. Augustine, Florida.)
February 2019 to January 2020: Drafting of document (higher-intensity volunteer time period)
May 2020 to July 2020: Voting period for draft document
August 2020 to December 2020: Revision of draft based on comments received during voting
September 2021: Expected publication date

Due date for submission of required documentation

Please submit nominations and required documentation (see Requirements for Nomination Consideration) on or before August 31, 2018 to