USA—The Clinical and Laboratory Standards Institute (CLSI) has published the second edition of M54—Principles and Procedures for Detection and Culture of Fungi in Clinical Specimens. This guideline describes recommended processes for plating and examining fungal cultures as well as principles and procedures for the direct detection of fungi in clinical specimens, including criteria for performing and interpreting direct microscopic examinations. Safety considerations unique to mycology laboratories and a discussion of appropriate levels of laboratory service (eg, when to refer samples to more experienced laboratories) are highlighted.
M54 also provides information on:
Fungal taxonomy has been updated in this edition of M54.
Nancy L. Wengenack, PhD, D(ABMM), Chairholder of M54, said, “The second edition of M54 builds on the strengths of the original document by adding additional information in the form of high-quality photographs and expanded tables designed to assist laboratorians with the interpretation of direct fungal microscopic examinations. In addition, information about the direct detection of fungi using antigen, antibody, and molecular methods expands the coverage of the document to these important areas of mycology. The revised document also updates taxonomy that has changed since the original publication.”
For more information about M54, contact Patrick McGinn at email@example.com or +1.484.588.5933.
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