CLSI C42
Erythrocyte Protoporphyrin Testing
CLSI C42 provides comprehensive guidance for accurate and standardized erythrocyte protoporphyrin (EP) measurement, reducing inconsistencies in current testing practices. This guideline covers the biochemistry and pathology of EP, summarizes its historical use, and defines its clinical applications. It recommends a specific molar absorptivity constant for standardization and emphasizes reporting EP as a molar ratio to heme. Detailed hematofluorometry and extraction methods for EP measurement are included, along with guidance on interpreting test results to enhance clinical decision-making.
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{{FormatPrice(nonMemberPrice)}} List PriceErythrocyte Protoporphyrin Testing; Approved Guideline (CLSI document C42-A) is a comprehensive document for use by laboratorians who perform erythrocyte protoporphyrin (EP) determinations; its aim is to reduce/eliminate the lack of uniformity in current measurement practices. The biochemistry and pathology of EP are discussed, the history of EP determinations is summarized, and the applications of the test are defined. The document recommends the adoption of a specific molar absorptivity constant for the standardization of EP methods and the universal adoption of reporting units expressed as the molar ratio of protoporphyrin to heme. Detailed methods for the measurement of EP by extraction and hematofluorometry are included, and the interpretation of EP results is discussed.
This document is useful to all laboratories that perform EP tests; users of hematofluorometry in nonlaboratory settings will benefit from its use as well.
Recommended methods for extraction (Section 8) and hematofluorometry (Section 9) of EP test procedures are given. The millimolar absorptivity (m?) of protoporphyrin IX (PPIX) is redefined (Section 7). This will result in changes in calibration and reference intervals for EP. New reference intervals are given (Section 10). The history of method development for EP (Section 5) and the biochemistry of porphyrins (Section 4) are described. Terminology (Section 4), sections on the causes of elevated porphyrin results (Section 6) and interpretation (Section 10), and a brief description of liquid chromatography for the differentiation of the various porphyrins (Section 8.9) are provided. The appendices address the preparation of QA materials and the availability of proficiency-testing programs.
Although NCCLS documents generally use units that are fully acceptable within the Système International d’Unités (SI), these do not always coincide with the units recommended by the International Union of Pure and Applied Chemistry (IUPAC) and by the International Federation of Clinical Chemistry (IFCC) for reporting results of clinical laboratory measurements. NCCLS documents also include the IUPAC/ IFCC-recommended units of volume (L) and substance (molecular) concentration (mol/L) in parentheses, where appropriate.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
This document is available in electronic format only.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
Erythrocyte Protoporphyrin Testing; Approved Guideline (CLSI document C42-A) is a comprehensive document for use by laboratorians who perform erythrocyte protoporphyrin (EP) determinations; its aim is to reduce/eliminate the lack of uniformity in current measurement practices. The biochemistry and pathology of EP are discussed, the history of EP determinations is summarized, and the applications of the test are defined. The document recommends the adoption of a specific molar absorptivity constant for the standardization of EP methods and the universal adoption of reporting units expressed as the molar ratio of protoporphyrin to heme. Detailed methods for the measurement of EP by extraction and hematofluorometry are included, and the interpretation of EP results is discussed.
This document is useful to all laboratories that perform EP tests; users of hematofluorometry in nonlaboratory settings will benefit from its use as well.
Recommended methods for extraction (Section 8) and hematofluorometry (Section 9) of EP test procedures are given. The millimolar absorptivity (m?) of protoporphyrin IX (PPIX) is redefined (Section 7). This will result in changes in calibration and reference intervals for EP. New reference intervals are given (Section 10). The history of method development for EP (Section 5) and the biochemistry of porphyrins (Section 4) are described. Terminology (Section 4), sections on the causes of elevated porphyrin results (Section 6) and interpretation (Section 10), and a brief description of liquid chromatography for the differentiation of the various porphyrins (Section 8.9) are provided. The appendices address the preparation of QA materials and the availability of proficiency-testing programs.
Although NCCLS documents generally use units that are fully acceptable within the Système International d’Unités (SI), these do not always coincide with the units recommended by the International Union of Pure and Applied Chemistry (IUPAC) and by the International Federation of Clinical Chemistry (IFCC) for reporting results of clinical laboratory measurements. NCCLS documents also include the IUPAC/ IFCC-recommended units of volume (L) and substance (molecular) concentration (mol/L) in parentheses, where appropriate.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
This document is available in electronic format only.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.