CLSI H07
Procedure for Determining Packed Cell Volume by the Microhematocrit Method
This standard outlines guidance for the microhematocrit method for measuring packed-cell volume, along with expert recommended materials and potential sources of error. This document is exclusively available in an electronic format for your convenience and accessibility.
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{{FormatPrice(nonMemberPrice)}} List PriceClinical and Laboratory Standards Institute document H07-A3—Procedure for Determining Packed Cell Volume by the Microhematocrit Method describes a standard method for direct measurement of packed cell volume (PCV). The standard is intended for reference use by clinical laboratory personnel and by manufacturers of instruments that determine PCV. The method can also be used (with appropriate precautions as described in the document) in the clinical laboratory for diagnostic purposes, for monitoring a patient's response to therapy, and for evaluating instruments and other methods for determining PCV; the standard should be used for whole blood calibration procedures of hematology analyzers. The document gives detailed specifications of the materials to be used in the procedure, contains information for calibrating the centrifuge and reading device, and includes information on verification of calibration. Expression of results, generally accepted reference values, and potential sources of error are given.
This document describes the determination of the packed (red) cell volume by centrifugation.
Determination of the PCV by centrifugation is:
• required for whole blood calibration of instrumental methods;
• applicable in evaluating instruments and alternative methods for determining PCV;
• applicable in the routine hematology laboratory (with appropriate precautions as described in the document) for diagnostic purposes and for monitoring progress of therapy, especially when the nature of the sample, e.g., presence of cold agglutinins, may cause inaccuracies in the (automated) method in routine use.
NOTE: Commercially available microhematocrit systems that use capillary tubes not made of glass, smaller volumes of blood, different centrifugation times, or different centrifugation speeds may produce results that are fully acceptable for diagnostic or screening purposes, or for therapy monitoring. (See Sections 5.2, 6.2, and 6.4 for additional information.) Manufacturers’ instructions must be followed for specific systems.
Thus, this standard is useful for all clinical laboratory personnel and for manufacturers of instruments that determine PCV.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
This document is available in electronic format only.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
Clinical and Laboratory Standards Institute document H07-A3—Procedure for Determining Packed Cell Volume by the Microhematocrit Method describes a standard method for direct measurement of packed cell volume (PCV). The standard is intended for reference use by clinical laboratory personnel and by manufacturers of instruments that determine PCV. The method can also be used (with appropriate precautions as described in the document) in the clinical laboratory for diagnostic purposes, for monitoring a patient's response to therapy, and for evaluating instruments and other methods for determining PCV; the standard should be used for whole blood calibration procedures of hematology analyzers. The document gives detailed specifications of the materials to be used in the procedure, contains information for calibrating the centrifuge and reading device, and includes information on verification of calibration. Expression of results, generally accepted reference values, and potential sources of error are given.
This document describes the determination of the packed (red) cell volume by centrifugation.
Determination of the PCV by centrifugation is:
• required for whole blood calibration of instrumental methods;
• applicable in evaluating instruments and alternative methods for determining PCV;
• applicable in the routine hematology laboratory (with appropriate precautions as described in the document) for diagnostic purposes and for monitoring progress of therapy, especially when the nature of the sample, e.g., presence of cold agglutinins, may cause inaccuracies in the (automated) method in routine use.
NOTE: Commercially available microhematocrit systems that use capillary tubes not made of glass, smaller volumes of blood, different centrifugation times, or different centrifugation speeds may produce results that are fully acceptable for diagnostic or screening purposes, or for therapy monitoring. (See Sections 5.2, 6.2, and 6.4 for additional information.) Manufacturers’ instructions must be followed for specific systems.
Thus, this standard is useful for all clinical laboratory personnel and for manufacturers of instruments that determine PCV.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
This document is available in electronic format only.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.