Standard Document
Second Edition
Immunology and Ligand Assay

CLSI ILA21

Clinical Evaluation of Immunoassays

This document provides essential guidance for the clinical evaluation of new immunoassays and expanded applications of existing assays. It supports assay developers, regulatory professionals, and clinical laboratories in designing and executing evaluations to ensure accuracy, regulatory compliance, and successful commercialization.

August 29, 2008
Marilyn M. Lightfoote, MD, PhD

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Abstract

Clinical and Laboratory Standards Institute document I/LA21-A2—Clinical Evaluation of Immunoassays; Approved Guideline—Second Edition addresses all aspects of the clinical evaluation of immunoassays developed for commercial or in-house use. Existing CLSI documents provide guidance for assessing analytical performance, methods comparison, and clinical accuracy of laboratory tests. This document focuses on unique characteristics of immunoassays, and provides a guide to designing, executing, and analyzing a clinical evaluation. In addition, this document will aid developers of “in-house” assays for institutional use, developers of assays used for monitoring pharmacologic effects of new drugs or biologics, and clinical and regulatory personnel responsible for commercializing products. The elements of this guideline include: 1) a development plan for an effective analysis and evaluation; 2) a discussion of the planning and design considerations that are necessary for a successful evaluation; 3) a description of requirements for conducting the evaluation through monitoring and database management; and 4) a brief review of the analytical performance measures that must be in place before testing clinical specimens.

Overview of Changes
During the revision process, the working group updated the content and expanded the current document for assessment of immunoassays to include specific details on selection and use of test specimen panels; specimen library collections; reference panels including specimen commutability issues; and sample size considerations for evaluation studies. An appendix was also added to guide the user in sample size selections. Numerous revisions were made to enhance and ensure global applications.
Scope
This document provides specific recommendations for the clinical evaluation of new immunoassays and new applications of existing assays, as well as multiple assay formats and their uses. It focuses on unique characteristics of immunoassays, and provides a guide to designing, executing, and analyzing a clinical evaluation. The intended users of this guideline are developers of “in-house” assays for institutional use, developers of assays used for monitoring pharmacologic effects of new drugs or biologics, and clinical and regulatory personnel responsible for commercializing products.
Product Details
ILA21A2E
1-56238-674-3
68
Additional Details

This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.

This document is available in electronic format only.

The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.

Authors
Marilyn M. Lightfoote, MD, PhD
Dorothy J. Ball, PhD
W. Harry Hannon, PhD
John C. Ridderhof, DrPH
Robert F. Vogt, Jr., PhD
Abstract

Clinical and Laboratory Standards Institute document I/LA21-A2—Clinical Evaluation of Immunoassays; Approved Guideline—Second Edition addresses all aspects of the clinical evaluation of immunoassays developed for commercial or in-house use. Existing CLSI documents provide guidance for assessing analytical performance, methods comparison, and clinical accuracy of laboratory tests. This document focuses on unique characteristics of immunoassays, and provides a guide to designing, executing, and analyzing a clinical evaluation. In addition, this document will aid developers of “in-house” assays for institutional use, developers of assays used for monitoring pharmacologic effects of new drugs or biologics, and clinical and regulatory personnel responsible for commercializing products. The elements of this guideline include: 1) a development plan for an effective analysis and evaluation; 2) a discussion of the planning and design considerations that are necessary for a successful evaluation; 3) a description of requirements for conducting the evaluation through monitoring and database management; and 4) a brief review of the analytical performance measures that must be in place before testing clinical specimens.

Overview of Changes
During the revision process, the working group updated the content and expanded the current document for assessment of immunoassays to include specific details on selection and use of test specimen panels; specimen library collections; reference panels including specimen commutability issues; and sample size considerations for evaluation studies. An appendix was also added to guide the user in sample size selections. Numerous revisions were made to enhance and ensure global applications.
Scope
This document provides specific recommendations for the clinical evaluation of new immunoassays and new applications of existing assays, as well as multiple assay formats and their uses. It focuses on unique characteristics of immunoassays, and provides a guide to designing, executing, and analyzing a clinical evaluation. The intended users of this guideline are developers of “in-house” assays for institutional use, developers of assays used for monitoring pharmacologic effects of new drugs or biologics, and clinical and regulatory personnel responsible for commercializing products.
Additional Details

This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.

This document is available in electronic format only.

The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.

Authors
Marilyn M. Lightfoote, MD, PhD
Dorothy J. Ball, PhD
W. Harry Hannon, PhD
John C. Ridderhof, DrPH
Robert F. Vogt, Jr., PhD