CLSI VET01
Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals
Keep your lab at the forefront of testing! CLSI VET01 Ed6 delivers the highest quality standards for current recommended methods for disk diffusion susceptibility testing. This guide provides easy reference methods for dilution and determination of minimal inhibitory concentrations for aerobic bacteria, navigating veterinary labs towards efficent and accurate treatment methods.
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{{FormatPrice(nonMemberPrice)}} List PriceAntimicrobial susceptibility testing is indicated for any organism that contributes to an infectious process warranting antimicrobial chemotherapy if its susceptibility cannot be reliably predicted from knowledge of the organism’s identity. Susceptibility tests are most often indicated when the causative organism is thought to belong to a species capable of exhibiting resistance to commonly used antimicrobial agents.
Various laboratory methods can be used to measure the in vitro susceptibility of bacteria to antimicrobial agents. Clinical and Laboratory Standards Institute VET01—Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals describes disk diffusion, as well as standard broth dilution (macrodilution and microdilution) and agar dilution, and it includes a series of procedures to standardize the way the tests are performed. The performance, applications, and limitations of the current CLSI-recommended methods are also described. The supplemental information (CLSI VET01S tables) used with this standard represents the most current information for antimicrobial agent selection, interpretation, and QC using the procedures standardized in CLSI VET01.
This standard describes reference agar disk diffusion techniques, as well as standard broth (macrodilution and microdilution) and agar dilution methods used to determine in vitro antimicrobial susceptibility of bacteria that grow aerobically.
It includes:
• Agar plate preparation
• Broth and agar dilution test preparation
• Testing conditions, including inoculum preparation and standardization, incubation time, and incubation temperature
• Results interpretation and reporting considerations
• QC procedures
• Disk diffusion and dilution test method limitations
To assist the veterinary laboratory, suggestions are provided for selecting antimicrobial agents for routine testing and reporting. Additionally, a brief overview of the various antimicrobial classes, bacterial mechanisms of antimicrobial resistance (AMR), and specific tests for detecting AMR are included.
For additional resources, standards for testing the in vitro antimicrobial susceptibility of bacteria isolated from humans that grow aerobically using disk or dilution methods are found in CLSI M100, M02, and M07. Standards for testing the in vitro antimicrobial susceptibility of bacteria that grow anaerobically are found in CLSI M11. Guidelines for standardized antimicrobial susceptibility testing (AST) of bacterial pathogens from aquatic animals are available in CLSI VET03. Guidelines for AST of infrequently isolated or fastidious bacteria that are not included in CLSI M100, M02, M07, or M11 are available in CLSI VET06 and CLSI M45. The AST methods provided in this standard can be used in laboratories around the world, including but not limited to:
• Veterinary and medical diagnostic laboratories
• Public health laboratories
• Research laboratories
• Food laboratories
• Environmental laboratories
This standard and its supplement (CLSI VET01S) are not intended to guide the use of antimicrobial agents that are used for production or disease prevention purposes.
Antimicrobial susceptibility testing is indicated for any organism that contributes to an infectious process warranting antimicrobial chemotherapy if its susceptibility cannot be reliably predicted from knowledge of the organism’s identity. Susceptibility tests are most often indicated when the causative organism is thought to belong to a species capable of exhibiting resistance to commonly used antimicrobial agents.
Various laboratory methods can be used to measure the in vitro susceptibility of bacteria to antimicrobial agents. Clinical and Laboratory Standards Institute VET01—Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals describes disk diffusion, as well as standard broth dilution (macrodilution and microdilution) and agar dilution, and it includes a series of procedures to standardize the way the tests are performed. The performance, applications, and limitations of the current CLSI-recommended methods are also described. The supplemental information (CLSI VET01S tables) used with this standard represents the most current information for antimicrobial agent selection, interpretation, and QC using the procedures standardized in CLSI VET01.
This standard describes reference agar disk diffusion techniques, as well as standard broth (macrodilution and microdilution) and agar dilution methods used to determine in vitro antimicrobial susceptibility of bacteria that grow aerobically.
It includes:
• Agar plate preparation
• Broth and agar dilution test preparation
• Testing conditions, including inoculum preparation and standardization, incubation time, and incubation temperature
• Results interpretation and reporting considerations
• QC procedures
• Disk diffusion and dilution test method limitations
To assist the veterinary laboratory, suggestions are provided for selecting antimicrobial agents for routine testing and reporting. Additionally, a brief overview of the various antimicrobial classes, bacterial mechanisms of antimicrobial resistance (AMR), and specific tests for detecting AMR are included.
For additional resources, standards for testing the in vitro antimicrobial susceptibility of bacteria isolated from humans that grow aerobically using disk or dilution methods are found in CLSI M100, M02, and M07. Standards for testing the in vitro antimicrobial susceptibility of bacteria that grow anaerobically are found in CLSI M11. Guidelines for standardized antimicrobial susceptibility testing (AST) of bacterial pathogens from aquatic animals are available in CLSI VET03. Guidelines for AST of infrequently isolated or fastidious bacteria that are not included in CLSI M100, M02, M07, or M11 are available in CLSI VET06 and CLSI M45. The AST methods provided in this standard can be used in laboratories around the world, including but not limited to:
• Veterinary and medical diagnostic laboratories
• Public health laboratories
• Research laboratories
• Food laboratories
• Environmental laboratories
This standard and its supplement (CLSI VET01S) are not intended to guide the use of antimicrobial agents that are used for production or disease prevention purposes.