Standard Document
Third Edition
Veterinary Medicine

CLSI VET04

Performance Standards for Antimicrobial Susceptibility Testing of Bacteria Isolated From Aquatic Animals

CLSI VET04 is a complete up-to-date guide featuring best practices for veterinary antimicrobial susceptibility (AST) testing guidance. This guide uses updated tables, tesing methodologies and procedures for bateria isolated from aquatic animals to assit veterinarians in making informed decisions for animal patient care.

The tables in VET04 must be used alongside VET03 to be valid.

May 20, 2020
Brian V. Lubbers, DVM, PhD, DACVCP

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Abstract

The data in the tables are valid only if the methodologies in CLSI document VET03 are followed. This guideline contains information about disk and broth dilution susceptibility test procedures for bacteria isolated from aquatic animals. The clinical importance of antimicrobial susceptibility test results demands that these tests be performed under optimal conditions and that laboratories have the capability to interpret results based on the most current clinical breakpoint or epidemiological cutoff value interpretive categories. The tables presented in VET04 represent the most current information for drug selection, interpretation quality control using the procedures standardized in VET031. Users should replace previously published tables with these new tables. Changes in the tables since the previous editions appear in boldface type. Users should consider the interpretive categories presented in these tables most useful to isolates of Aeromonas salmonicida, Aeromonas hydrophila, Flavobacterium columnare, and Flavobacterium psychrophilum. Careful extrapolations may be possible to other bacterial species and with other similar antimicrobial agents, but only after consulting CLSI document VET09. Fish disease diagnostic laboratories that typically conduct susceptibility testing less often than once per week should consult this document for revised guidance for frequency of QC.

Product Details
VET04Ed3E
978-1-68440-076-8
88
Authors
Ron A. Miller, MS, PhD
Charles M. Gieseker, MS, PhD
Ruben Avendaño-Herrera, PhD
Sandrine Baron, PhD
Nicky Buller, PhD, BSc
Claire R. Burbick, DVM, PhD, DACVM
Rungtip Chuanchuen, DVM, MS, PhD
Inger Dalsgaard, DVM, PhD
Annelies M. Declercq, PhD, MSc
Patricia S. Gaunt, DVM, PhD, DABVT
John P. Hawke, PhD
Hui-Min Hsu, DVM, MS, PhD
Renate Reimschuessel, VMD, PhD
Esteban Soto, Med Vet, MSc, PhD, DACVM
Peter R. Smith, BA, PhD
David Verner-Jeffreys, PhD, MSc, BSc
Ching Ching Wu, DVM
Abstract

The data in the tables are valid only if the methodologies in CLSI document VET03 are followed. This guideline contains information about disk and broth dilution susceptibility test procedures for bacteria isolated from aquatic animals. The clinical importance of antimicrobial susceptibility test results demands that these tests be performed under optimal conditions and that laboratories have the capability to interpret results based on the most current clinical breakpoint or epidemiological cutoff value interpretive categories. The tables presented in VET04 represent the most current information for drug selection, interpretation quality control using the procedures standardized in VET031. Users should replace previously published tables with these new tables. Changes in the tables since the previous editions appear in boldface type. Users should consider the interpretive categories presented in these tables most useful to isolates of Aeromonas salmonicida, Aeromonas hydrophila, Flavobacterium columnare, and Flavobacterium psychrophilum. Careful extrapolations may be possible to other bacterial species and with other similar antimicrobial agents, but only after consulting CLSI document VET09. Fish disease diagnostic laboratories that typically conduct susceptibility testing less often than once per week should consult this document for revised guidance for frequency of QC.

VET04Ed3E
978-1-68440-076-8
88
Authors
Ron A. Miller, MS, PhD
Charles M. Gieseker, MS, PhD
Ruben Avendaño-Herrera, PhD
Sandrine Baron, PhD
Nicky Buller, PhD, BSc
Claire R. Burbick, DVM, PhD, DACVM
Rungtip Chuanchuen, DVM, MS, PhD
Inger Dalsgaard, DVM, PhD
Annelies M. Declercq, PhD, MSc
Patricia S. Gaunt, DVM, PhD, DABVT
John P. Hawke, PhD
Hui-Min Hsu, DVM, MS, PhD
Renate Reimschuessel, VMD, PhD
Esteban Soto, Med Vet, MSc, PhD, DACVM
Peter R. Smith, BA, PhD
David Verner-Jeffreys, PhD, MSc, BSc
Ching Ching Wu, DVM