CLSI VET04
Performance Standards for Antimicrobial Susceptibility Testing of Bacteria Isolated From Aquatic Animals
CLSI VET04 is a complete up-to-date guide featuring best practices for veterinary antimicrobial susceptibility (AST) testing guidance. This guide uses updated tables, tesing methodologies and procedures for bateria isolated from aquatic animals to assit veterinarians in making informed decisions for animal patient care.
The tables in VET04 must be used alongside VET03 to be valid.
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{{FormatPrice(nonMemberPrice)}} List PriceThe data in the tables are valid only if the methodologies in CLSI document VET03 are followed. This guideline contains information about disk and broth dilution susceptibility test procedures for bacteria isolated from aquatic animals. The clinical importance of antimicrobial susceptibility test results demands that these tests be performed under optimal conditions and that laboratories have the capability to interpret results based on the most current clinical breakpoint or epidemiological cutoff value interpretive categories. The tables presented in VET04 represent the most current information for drug selection, interpretation quality control using the procedures standardized in VET031. Users should replace previously published tables with these new tables. Changes in the tables since the previous editions appear in boldface type. Users should consider the interpretive categories presented in these tables most useful to isolates of Aeromonas salmonicida, Aeromonas hydrophila, Flavobacterium columnare, and Flavobacterium psychrophilum. Careful extrapolations may be possible to other bacterial species and with other similar antimicrobial agents, but only after consulting CLSI document VET09. Fish disease diagnostic laboratories that typically conduct susceptibility testing less often than once per week should consult this document for revised guidance for frequency of QC.
This supplement replaces the previous edition of the supplement VET03/VET04-S2, published in 2014. This list includes the major changes in this document. Other minor or editorial changes were made to the general formatting and to some of the table footnotes and comments. Changes to the tables since the previous edition appear in boldface type. The following are additions or changes unless otherwise noted as a "deletion."
• General:
– Changed document code from VET03/VET04 to VET04 to differentiate it from the methods guideline, VET031
? NOTE: The broth dilution and disk diffusion methods, formerly in separate CLSI guidelines (VET03 and VET04, respectively) have been combined into one methods guideline, VET03.1
? Harmonized language and common information on methods and QC with CLSI documents VET01,4 VET08,5 M02,6 the M02 Disk Diffusion Reading Guide,7 M07,8 and M100.9
– Updated genera formerly included in the family Enterobacteriaceae reorganized to an order (Enterobacterales) containing seven families: Budviciaceae, Enterobacteriaceae, Erwiniaceae, Hafniaceae, Morganellaceae, Pectobacteriaceae, Yersiniaceae10
– To align with the International Organization for Standardization, changed the name of the inoculum preparation method in all appropriate tables from growth method to broth culture method and changed direct colony suspension to colony suspension
– Reorganized and reformatted tables:
? Breakpoint table for Aeromonas salmonicida (Table 1)
? Epidemiological cutoff value (ECV) tables (Tables 2A through 2D)
? QC tables with minimal inhibitory concentration (MIC) QC ranges (Tables 4A through 4F)
? QC tables with disk diffusion QC ranges (Tables 5A through 5D)
• Summary of CLSI Processes for Establishing Breakpoints and Quality Control Ranges (p. xv):
– Added new section
• CLSI Reference Methods vs Commercial Methods and CLSI vs Regulatory Authority (p. xvi):
– Added new section
• CLSI Aquatic Animal–Specific Breakpoint Additions/Revisions Since 2008 (p. xvii):
– Added new table of breakpoint additions and revisions since 2008, organized in alphabetical order by antimicrobial agent
• CLSI Aquaculture Pathogen–Specific Epidemiological Cutoff Value Additions/Revisions Since 2008 (p. xvii):
– Added new table of ECV additions and revisions since 2008, organized in order of appearance in the tables by organism group (2A, 2B, 2C, 2D), then in alphabetical order by antimicrobial agent
• Subcommittee on Veterinary Antimicrobial Susceptibility Testing Mission Statement and Responsibilities (p. xviii):
– Added new section
• Instructions for Use of Tables:
– Added new section with general instructions for using the tables, including:
? Selecting antimicrobial agents for testing and reporting (p. 1)
? Breakpoints and interpretive category definitions (p. 2)
? Epidemiological cutoff values and interpretive category definitions (p. 4)
? Reporting results (p. 4)
? Therapy-related comments (p. 5)
? Confirmation of animal results (p. 5)
? Development of resistance and testing of repeat isolates (p. 5)
? QC overview (p. 6)
? Abbreviations and acronyms (p. 6)
• Table 1. MIC and Zone Diameter Breakpoints for Aeromonas salmonicida:
– Added:
? References to Tables 4B and 5B for acceptable QC ranges (p. 8)
? Reference to CLSI document VET092 in general comments (p. 8)
? References for Table 1 (p. 10)
– Clarified:
? Medium used for broth dilution and disk diffusion (p. 8)
– Revised:
? General comment to refer to Tables 4B and 5B (p. 8)
– Deleted:
? Information in general comments about ECVs used in support of setting breakpoints
• Table 2A. MIC and Zone Diameter Epidemiological Cutoff Values for Aeromonas salmonicida:
– Added:
? References to Tables 4B and 5B for acceptable QC ranges (p. 12)
? Statistical methods (ECOFFinder and normalized resistance interpretation) and references to general comment (p. 12)
? References for Table 2A (p. 14)
– Clarified:
? Medium used for broth dilution and disk diffusion (p. 12)
– Revised:
? General comment to refer to Tables 4B and 5B (p. 12)
? General comment to clarify that:
¦ ECVs were initially established based on visual inspection and on error-rate bounding of MIC and/or zone diameter data associated with an epidemiological database derived from geographically diverse diagnostic laboratory surveys (p. 12).
¦ The same database from the initial study was reanalyzed in 2019 using two statistical methods (p. 12).
• Table 2B. MIC and Zone Diameter Epidemiological Cutoff Values for Aeromonas hydrophila:
– Added new table that includes:
? Testing conditions with medium used for broth dilution and disk diffusion testing (p. 16)
? QC recommendations (p. 16)
? Interpretive categories and zone diameter and MIC ECVs for:
¦ Enrofloxacin (p. 17)
¦ Erythromycin (MIC ECVs only) (p. 17)
¦ Florfenicol (p. 17)
¦ Gentamicin (p. 17)
¦ Oxolinic acid (p. 17)
¦ Oxytetracycline (p. 17)
? Comment indicating all ECVs were established based on visual inspection and statistical analysis of zone diameter and MIC distributions of 104 A. hydrophila isolates (p. 17)
• Table 2C. MIC Epidemiological Cutoff Values for Flavobacterium columnare:
– Added new table that includes:
? Testing conditions with medium used for broth dilution testing (p. 18)
? QC recommendations (p. 18)
? Interpretive categories and MIC ECVs for:
¦ Ampicillin (p. 19)
¦ Enrofloxacin (p. 19)
¦ Erythromycin (p. 19)
¦ Florfenicol (p. 19)
¦ Flumequine (p. 19)
¦ Oxolinic acid (p. 19)
¦ Oxytetracycline (p. 19)
? Comment indicating all ECVs were established based on visual inspection and statistical analysis of MIC distributions of 229 F. columnare isolates (p. 19)
• Table 2D. MIC Epidemiological Cutoff Values for Flavobacterium psychrophilum:
– Added new table that includes:
? Testing conditions with medium used for broth dilution testing (p. 20)
? QC recommendations (p. 20)
? Interpretive categories and MIC ECVs for:
¦ Enrofloxacin (p. 21)
¦ Erythromycin (p. 21)
¦ Florfenicol (p. 21)
¦ Flumequine (p. 21)
¦ Oxolinic acid (p. 21)
¦ Oxytetracycline (p. 21)
? Comments indicating ECVs for:
¦ Enrofloxacin, erythromycin, flumequine, and oxolinic acid were established based on visual inspection and statistical analysis of MIC distributions of 291 F. psychrophilum isolates (p. 21)
¦ Florfenicol were established based on visual inspection and statistical analysis of MIC distributions of 353 F. psychrophilum isolates (p. 21)
¦ Oxytetracycline were established based on visual inspection and statistical analysis of MIC distributions of 325 F. psychrophilum isolates (p. 21)
• Table 3. QC Strain Culture Collection Numbers for Antimicrobial Susceptibility Tests (p. 24):
– Added new table with QC strain cultures for aquaculture antimicrobial susceptibility testing from CLSI document VET08,5 with the addition of A. salmonicida subsp. salmonicida ATCC® 33658 and an additional source, the National Collection of Industrial Food and Marine Bacteria
• Tables 4A through 4F. MIC QC Ranges (pp. 26–39):
– Reorganized and renumbered all MIC QC tables (formerly Tables 6 through 11)
• Tables 4A (p. 26), 4B (p. 28), 4C (p. 30), and 4E (p. 36):
– Added new reference for A-layer phenotype information included in footnote
• Table 4D. MIC QC Ranges for Testing at 35°C ± 2°C (p. 32):
– Added or changed QC ranges for:
? Staphylococcus aureus ATCC® 29213
¦ Cefoperazone
¦ Cephalexin
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Monensin
¦ Narasin
¦ Piperacillin-tazobactam
¦ Tildipirosin
? Enterococcus faecalis ATCC® 29212
¦ Avilamycin
¦ Gamithromycin
¦ Monensin
¦ Narasin
¦ Piperacillin-tazobactam
? Escherichia coli ATCC® 25922
¦ Cefoperazone
¦ Cephalexin
¦ Kanamycin-cephalexin
¦ Piperacillin-tazobactam
¦ Tildipirosin
? Pseudomonas aeruginosa ATCC® 27853
¦ Cefoperazone
¦ Piperacillin-tazobactam
? Streptococcus pneumoniae ATCC® 49619
¦ Amoxicillin (added footnote)
¦ Gamithromycin
¦ Kanamycin-cephalexin
– Added:
? Footnote to MIC QC ranges with the media to be used, except as noted
? Footnote to amoxicillin QC range for S. pneumoniae ATCC® 49619 with information about the approved testing method and media supplementation
? Footnote to amoxicillin-clavulanate and piperacillin-tazobactam with additional QC ranges for E. coli ATCC® 35218 and cross-reference to information in CLSI document M1009
? Footnote to fosfomycin with information about the approved testing method and media supplementation
? Cross-reference to information in CLSI document VET014 about QC ranges for Mannheimia haemolytica ATCC® 33396
? New note with information about determining whether Mueller-Hinton agar (MHA) contains excessive levels of thymidine or thymine, QC organisms, and testing instructions
• Table 4F. MIC QC Ranges for Testing at 28°C ± 2°C (44 to 48 Hours) in Diluted Cation-Adjusted Mueller-Hinton Broth (p. 38):
– Added new footnote to A. salmonicida subsp. salmonicida ATCC® 33658 about A-layer phenotype of various culture collection isolates and reference
• Tables 5A through 5D. Disk Diffusion QC Ranges (pp. 40–48):
– Reorganized and renumbered all disk diffusion QC tables (formerly in CLSI document VET03-A, Tables 5 through 8)
• Tables 5A through 5C (pp. 40–44):
– Added new footnote to A. salmonicida subsp. salmonicida ATCC® 33658 about A-layer phenotype of various culture collection isolates and reference
• Table 5D. Disk Diffusion QC Ranges for Testing at 35°C ± 2°C (p. 46):
– Added or changed QC ranges for:
? E. coli ATCC® 25922
¦ Cefoperazone
¦ Cefovecin
¦ Cefquinome
¦ Doxycycline
¦ Kanamycin-cephalexin
¦ Neomycin
¦ Piperacillin
¦ Piperacillin-tazobactam
? S. aureus ATCC® 25923
¦ Cefoperazone
¦ Cefovecin
¦ Cefquinome
¦ Doxycycline
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Neomycin
¦ Piperacillin-tazobactam
¦ Tildipirosin
¦ Tulathromycin
? P. aeruginosa ATCC® 27853
¦ Piperacillin
¦ Piperacillin-tazobactam
? S. pneumoniae ATCC® 49619
¦ Cefovecin
¦ Cefquinome
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Tulathromycin
• Table 6. Solvents and Diluents for Preparing Stock Solutions of Antimicrobial Agents (p. 50):
– Reformatted (previously Table 4)
• Table 7. Preparing Dilutions of Antimicrobial Agents to Be Used in Broth Dilution Susceptibility Tests (p. 54):
– Reformatted (previously Table 5)
• Appendix A. Frequently Isolated Bacterial Pathogens of Fish and Shellfish (p. 56):
– Updated and relocated (previously Table 3)
• Appendix B. Conditions for Broth Dilution Antimicrobial Susceptibility Tests (p. 58):
– Table B1. Standardized Conditions for Broth Dilution Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 1 and 3) and Performance of QC:
? Reformatted, updated, and relocated (previously Table 1)
? Added incubation condition of ambient air for Group 1 and Group 3 organisms
? Added incubation conditions 35°C ± 2°C for 16–20 hours to applicable Group 1 organisms
– Table B2. Recommended Conditions Without QC Ranges Established for Developing Broth Dilution Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 2, 4, and 5):
? Reformatted, updated, and relocated (previously Table 2)
? Added incubation condition of ambient air for all organisms except Mycobacterium spp. and Nocardia spp.
• Appendix C. Conditions for Disk Diffusion Antimicrobial Susceptibility Tests (p. 60):
– Table C1. Standardized Conditions for Disk Diffusion Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Group 1) and Performance of QC:
? Reformatted, updated, and relocated (previously in CLSI document VET03-A, Table 3)
? Added incubation condition of ambient air to all Group 1 organisms except nonfastidious gram-positive cocci
? Added ± 2°C to all incubation temperatures
? Added incubation conditions 35°C ± 2°C for all organisms except A. salmonicida
– Table C2. Recommended Conditions Without QC Ranges Established for Developing Disk Diffusion Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 2, 3, 4, and 5):
? Reformatted, updated, and relocated (previously in CLSI document VET03-A, Table 4)
? Added incubation condition of ambient air to all Group 2 and Group 3 organisms and to psychrophilic A. salmonicida, Tenacibaculum maritimum, and Tenacibaculum dicentrarchi in Group 5
? Added ± 2°C to all incubation temperatures
The data in the tables are valid only if the methodologies in CLSI document VET03 are followed. This guideline contains information about disk and broth dilution susceptibility test procedures for bacteria isolated from aquatic animals. The clinical importance of antimicrobial susceptibility test results demands that these tests be performed under optimal conditions and that laboratories have the capability to interpret results based on the most current clinical breakpoint or epidemiological cutoff value interpretive categories. The tables presented in VET04 represent the most current information for drug selection, interpretation quality control using the procedures standardized in VET031. Users should replace previously published tables with these new tables. Changes in the tables since the previous editions appear in boldface type. Users should consider the interpretive categories presented in these tables most useful to isolates of Aeromonas salmonicida, Aeromonas hydrophila, Flavobacterium columnare, and Flavobacterium psychrophilum. Careful extrapolations may be possible to other bacterial species and with other similar antimicrobial agents, but only after consulting CLSI document VET09. Fish disease diagnostic laboratories that typically conduct susceptibility testing less often than once per week should consult this document for revised guidance for frequency of QC.
This supplement replaces the previous edition of the supplement VET03/VET04-S2, published in 2014. This list includes the major changes in this document. Other minor or editorial changes were made to the general formatting and to some of the table footnotes and comments. Changes to the tables since the previous edition appear in boldface type. The following are additions or changes unless otherwise noted as a "deletion."
• General:
– Changed document code from VET03/VET04 to VET04 to differentiate it from the methods guideline, VET031
? NOTE: The broth dilution and disk diffusion methods, formerly in separate CLSI guidelines (VET03 and VET04, respectively) have been combined into one methods guideline, VET03.1
? Harmonized language and common information on methods and QC with CLSI documents VET01,4 VET08,5 M02,6 the M02 Disk Diffusion Reading Guide,7 M07,8 and M100.9
– Updated genera formerly included in the family Enterobacteriaceae reorganized to an order (Enterobacterales) containing seven families: Budviciaceae, Enterobacteriaceae, Erwiniaceae, Hafniaceae, Morganellaceae, Pectobacteriaceae, Yersiniaceae10
– To align with the International Organization for Standardization, changed the name of the inoculum preparation method in all appropriate tables from growth method to broth culture method and changed direct colony suspension to colony suspension
– Reorganized and reformatted tables:
? Breakpoint table for Aeromonas salmonicida (Table 1)
? Epidemiological cutoff value (ECV) tables (Tables 2A through 2D)
? QC tables with minimal inhibitory concentration (MIC) QC ranges (Tables 4A through 4F)
? QC tables with disk diffusion QC ranges (Tables 5A through 5D)
• Summary of CLSI Processes for Establishing Breakpoints and Quality Control Ranges (p. xv):
– Added new section
• CLSI Reference Methods vs Commercial Methods and CLSI vs Regulatory Authority (p. xvi):
– Added new section
• CLSI Aquatic Animal–Specific Breakpoint Additions/Revisions Since 2008 (p. xvii):
– Added new table of breakpoint additions and revisions since 2008, organized in alphabetical order by antimicrobial agent
• CLSI Aquaculture Pathogen–Specific Epidemiological Cutoff Value Additions/Revisions Since 2008 (p. xvii):
– Added new table of ECV additions and revisions since 2008, organized in order of appearance in the tables by organism group (2A, 2B, 2C, 2D), then in alphabetical order by antimicrobial agent
• Subcommittee on Veterinary Antimicrobial Susceptibility Testing Mission Statement and Responsibilities (p. xviii):
– Added new section
• Instructions for Use of Tables:
– Added new section with general instructions for using the tables, including:
? Selecting antimicrobial agents for testing and reporting (p. 1)
? Breakpoints and interpretive category definitions (p. 2)
? Epidemiological cutoff values and interpretive category definitions (p. 4)
? Reporting results (p. 4)
? Therapy-related comments (p. 5)
? Confirmation of animal results (p. 5)
? Development of resistance and testing of repeat isolates (p. 5)
? QC overview (p. 6)
? Abbreviations and acronyms (p. 6)
• Table 1. MIC and Zone Diameter Breakpoints for Aeromonas salmonicida:
– Added:
? References to Tables 4B and 5B for acceptable QC ranges (p. 8)
? Reference to CLSI document VET092 in general comments (p. 8)
? References for Table 1 (p. 10)
– Clarified:
? Medium used for broth dilution and disk diffusion (p. 8)
– Revised:
? General comment to refer to Tables 4B and 5B (p. 8)
– Deleted:
? Information in general comments about ECVs used in support of setting breakpoints
• Table 2A. MIC and Zone Diameter Epidemiological Cutoff Values for Aeromonas salmonicida:
– Added:
? References to Tables 4B and 5B for acceptable QC ranges (p. 12)
? Statistical methods (ECOFFinder and normalized resistance interpretation) and references to general comment (p. 12)
? References for Table 2A (p. 14)
– Clarified:
? Medium used for broth dilution and disk diffusion (p. 12)
– Revised:
? General comment to refer to Tables 4B and 5B (p. 12)
? General comment to clarify that:
¦ ECVs were initially established based on visual inspection and on error-rate bounding of MIC and/or zone diameter data associated with an epidemiological database derived from geographically diverse diagnostic laboratory surveys (p. 12).
¦ The same database from the initial study was reanalyzed in 2019 using two statistical methods (p. 12).
• Table 2B. MIC and Zone Diameter Epidemiological Cutoff Values for Aeromonas hydrophila:
– Added new table that includes:
? Testing conditions with medium used for broth dilution and disk diffusion testing (p. 16)
? QC recommendations (p. 16)
? Interpretive categories and zone diameter and MIC ECVs for:
¦ Enrofloxacin (p. 17)
¦ Erythromycin (MIC ECVs only) (p. 17)
¦ Florfenicol (p. 17)
¦ Gentamicin (p. 17)
¦ Oxolinic acid (p. 17)
¦ Oxytetracycline (p. 17)
? Comment indicating all ECVs were established based on visual inspection and statistical analysis of zone diameter and MIC distributions of 104 A. hydrophila isolates (p. 17)
• Table 2C. MIC Epidemiological Cutoff Values for Flavobacterium columnare:
– Added new table that includes:
? Testing conditions with medium used for broth dilution testing (p. 18)
? QC recommendations (p. 18)
? Interpretive categories and MIC ECVs for:
¦ Ampicillin (p. 19)
¦ Enrofloxacin (p. 19)
¦ Erythromycin (p. 19)
¦ Florfenicol (p. 19)
¦ Flumequine (p. 19)
¦ Oxolinic acid (p. 19)
¦ Oxytetracycline (p. 19)
? Comment indicating all ECVs were established based on visual inspection and statistical analysis of MIC distributions of 229 F. columnare isolates (p. 19)
• Table 2D. MIC Epidemiological Cutoff Values for Flavobacterium psychrophilum:
– Added new table that includes:
? Testing conditions with medium used for broth dilution testing (p. 20)
? QC recommendations (p. 20)
? Interpretive categories and MIC ECVs for:
¦ Enrofloxacin (p. 21)
¦ Erythromycin (p. 21)
¦ Florfenicol (p. 21)
¦ Flumequine (p. 21)
¦ Oxolinic acid (p. 21)
¦ Oxytetracycline (p. 21)
? Comments indicating ECVs for:
¦ Enrofloxacin, erythromycin, flumequine, and oxolinic acid were established based on visual inspection and statistical analysis of MIC distributions of 291 F. psychrophilum isolates (p. 21)
¦ Florfenicol were established based on visual inspection and statistical analysis of MIC distributions of 353 F. psychrophilum isolates (p. 21)
¦ Oxytetracycline were established based on visual inspection and statistical analysis of MIC distributions of 325 F. psychrophilum isolates (p. 21)
• Table 3. QC Strain Culture Collection Numbers for Antimicrobial Susceptibility Tests (p. 24):
– Added new table with QC strain cultures for aquaculture antimicrobial susceptibility testing from CLSI document VET08,5 with the addition of A. salmonicida subsp. salmonicida ATCC® 33658 and an additional source, the National Collection of Industrial Food and Marine Bacteria
• Tables 4A through 4F. MIC QC Ranges (pp. 26–39):
– Reorganized and renumbered all MIC QC tables (formerly Tables 6 through 11)
• Tables 4A (p. 26), 4B (p. 28), 4C (p. 30), and 4E (p. 36):
– Added new reference for A-layer phenotype information included in footnote
• Table 4D. MIC QC Ranges for Testing at 35°C ± 2°C (p. 32):
– Added or changed QC ranges for:
? Staphylococcus aureus ATCC® 29213
¦ Cefoperazone
¦ Cephalexin
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Monensin
¦ Narasin
¦ Piperacillin-tazobactam
¦ Tildipirosin
? Enterococcus faecalis ATCC® 29212
¦ Avilamycin
¦ Gamithromycin
¦ Monensin
¦ Narasin
¦ Piperacillin-tazobactam
? Escherichia coli ATCC® 25922
¦ Cefoperazone
¦ Cephalexin
¦ Kanamycin-cephalexin
¦ Piperacillin-tazobactam
¦ Tildipirosin
? Pseudomonas aeruginosa ATCC® 27853
¦ Cefoperazone
¦ Piperacillin-tazobactam
? Streptococcus pneumoniae ATCC® 49619
¦ Amoxicillin (added footnote)
¦ Gamithromycin
¦ Kanamycin-cephalexin
– Added:
? Footnote to MIC QC ranges with the media to be used, except as noted
? Footnote to amoxicillin QC range for S. pneumoniae ATCC® 49619 with information about the approved testing method and media supplementation
? Footnote to amoxicillin-clavulanate and piperacillin-tazobactam with additional QC ranges for E. coli ATCC® 35218 and cross-reference to information in CLSI document M1009
? Footnote to fosfomycin with information about the approved testing method and media supplementation
? Cross-reference to information in CLSI document VET014 about QC ranges for Mannheimia haemolytica ATCC® 33396
? New note with information about determining whether Mueller-Hinton agar (MHA) contains excessive levels of thymidine or thymine, QC organisms, and testing instructions
• Table 4F. MIC QC Ranges for Testing at 28°C ± 2°C (44 to 48 Hours) in Diluted Cation-Adjusted Mueller-Hinton Broth (p. 38):
– Added new footnote to A. salmonicida subsp. salmonicida ATCC® 33658 about A-layer phenotype of various culture collection isolates and reference
• Tables 5A through 5D. Disk Diffusion QC Ranges (pp. 40–48):
– Reorganized and renumbered all disk diffusion QC tables (formerly in CLSI document VET03-A, Tables 5 through 8)
• Tables 5A through 5C (pp. 40–44):
– Added new footnote to A. salmonicida subsp. salmonicida ATCC® 33658 about A-layer phenotype of various culture collection isolates and reference
• Table 5D. Disk Diffusion QC Ranges for Testing at 35°C ± 2°C (p. 46):
– Added or changed QC ranges for:
? E. coli ATCC® 25922
¦ Cefoperazone
¦ Cefovecin
¦ Cefquinome
¦ Doxycycline
¦ Kanamycin-cephalexin
¦ Neomycin
¦ Piperacillin
¦ Piperacillin-tazobactam
? S. aureus ATCC® 25923
¦ Cefoperazone
¦ Cefovecin
¦ Cefquinome
¦ Doxycycline
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Neomycin
¦ Piperacillin-tazobactam
¦ Tildipirosin
¦ Tulathromycin
? P. aeruginosa ATCC® 27853
¦ Piperacillin
¦ Piperacillin-tazobactam
? S. pneumoniae ATCC® 49619
¦ Cefovecin
¦ Cefquinome
¦ Gamithromycin
¦ Kanamycin-cephalexin
¦ Tulathromycin
• Table 6. Solvents and Diluents for Preparing Stock Solutions of Antimicrobial Agents (p. 50):
– Reformatted (previously Table 4)
• Table 7. Preparing Dilutions of Antimicrobial Agents to Be Used in Broth Dilution Susceptibility Tests (p. 54):
– Reformatted (previously Table 5)
• Appendix A. Frequently Isolated Bacterial Pathogens of Fish and Shellfish (p. 56):
– Updated and relocated (previously Table 3)
• Appendix B. Conditions for Broth Dilution Antimicrobial Susceptibility Tests (p. 58):
– Table B1. Standardized Conditions for Broth Dilution Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 1 and 3) and Performance of QC:
? Reformatted, updated, and relocated (previously Table 1)
? Added incubation condition of ambient air for Group 1 and Group 3 organisms
? Added incubation conditions 35°C ± 2°C for 16–20 hours to applicable Group 1 organisms
– Table B2. Recommended Conditions Without QC Ranges Established for Developing Broth Dilution Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 2, 4, and 5):
? Reformatted, updated, and relocated (previously Table 2)
? Added incubation condition of ambient air for all organisms except Mycobacterium spp. and Nocardia spp.
• Appendix C. Conditions for Disk Diffusion Antimicrobial Susceptibility Tests (p. 60):
– Table C1. Standardized Conditions for Disk Diffusion Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Group 1) and Performance of QC:
? Reformatted, updated, and relocated (previously in CLSI document VET03-A, Table 3)
? Added incubation condition of ambient air to all Group 1 organisms except nonfastidious gram-positive cocci
? Added ± 2°C to all incubation temperatures
? Added incubation conditions 35°C ± 2°C for all organisms except A. salmonicida
– Table C2. Recommended Conditions Without QC Ranges Established for Developing Disk Diffusion Antimicrobial Susceptibility Tests for Aquatic Bacterial Pathogens (Groups 2, 3, 4, and 5):
? Reformatted, updated, and relocated (previously in CLSI document VET03-A, Table 4)
? Added incubation condition of ambient air to all Group 2 and Group 3 organisms and to psychrophilic A. salmonicida, Tenacibaculum maritimum, and Tenacibaculum dicentrarchi in Group 5
? Added ± 2°C to all incubation temperatures