CLSI C29
Standardization of Sodium and Potassium Ion-Selective Electrode Systems to the Flame Photometric Reference Method
CLSI C29 provides a protocol for standardizing direct ion-selective electrode (ISE) systems, ensuring their results are traceable to the reference flame photometry method for specimens with normal plasma water. This standard guides laboratories in verifying the accuracy of direct potentiometric instruments by detailing the preparation and use of serum pools for calibration. Laboratories can either prepare these pools themselves or obtain them from the National Institute of Standards and Technology (NIST). Ensuring consistent and accurate sodium and potassium measurements is essential for reliable clinical practice, and C29 provides the necessary framework to achieve this standardization.
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{{FormatPrice(nonMemberPrice)}} List PriceStandardization of Sodium and Potassium Ion-Selective Electrode Systems to the Flame Photometric Reference Method; Approved Standard—Second Edition (CLSI document C29-A2) offers a protocol for standardizing instruments that contain direct ion-selective electrodes to give results in concentration terms that are verifiable to the reference method (flame photometry) for specimens with normal plasma water (i.e., lipids and proteins). The document describes the preparation of serum pools to carry out the procedure. Laboratories without the resources, equipment, or personnel to prepare the pools can purchase them from the National Institute of Standards and Technology (Gaithersburg, MD). It is recommended in C29-A2 that the accuracy of each new direct potentiometric instrument be verified with these standard pools, together with the flame photometer, if it is also used to report patient results.
This document addresses the determination of sodium and potassium in undiluted plasma in routine clinical practice using ion-selective electrodes.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
This document is available in electronic format only.
Standardization of Sodium and Potassium Ion-Selective Electrode Systems to the Flame Photometric Reference Method; Approved Standard—Second Edition (CLSI document C29-A2) offers a protocol for standardizing instruments that contain direct ion-selective electrodes to give results in concentration terms that are verifiable to the reference method (flame photometry) for specimens with normal plasma water (i.e., lipids and proteins). The document describes the preparation of serum pools to carry out the procedure. Laboratories without the resources, equipment, or personnel to prepare the pools can purchase them from the National Institute of Standards and Technology (Gaithersburg, MD). It is recommended in C29-A2 that the accuracy of each new direct potentiometric instrument be verified with these standard pools, together with the flame photometer, if it is also used to report patient results.
This document addresses the determination of sodium and potassium in undiluted plasma in routine clinical practice using ion-selective electrodes.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
This document is available in electronic format only.