CLSI H44

Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes), 2nd Edition

This document provides guidance for the performance of reticulocyte counting by flow cytometry. It includes methods for determining the trueness and precision of the reticulocyte flow cytometry instrument and a recommended reference procedure.

This document is available in electronic format only.

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Chairholder: Charles F. Arkin, MD

Date of Publication: February 1, 2004

Order Code PDF: CLSI H44A2E
ISBN Number: 1-56238-527-5

Order Code Print: print not available

Edition: Second

Pages: 39

CLSI H44 Additional Details

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This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid as of September 2018. Because of its value to the laboratory community, it is being retained in CLSI’s library.

CLSI H44 Abstract

Clinical and Laboratory Standards Institute document H44-A2—Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry, and Supravital Dyes); Approved Guideline—Second Edition provides guidance for the performance of reticulocyte counting by flow cytometry and automated hematology instruments. This guideline addresses methods for determining the precision and trueness of the flow cytometer and blood cell counters based upon principles of focused flow dynamics, along with recommendations for calibration and quality control. A description of the new methylene blue (NMB) method, a method against which the test instrument can be compared, is also included. Additional topics discussed include reference intervals and use of related reticulocyte parameters, i.e., the immature reticulocyte fraction (formerly termed “reticulocyte maturation index”) and reticulocyte hemoglobin content (CHr).