H52

This guideline addresses the diagnostic red blood cell (RBC) assays performed as fluorescence-based assays on a flow cytometry platform; including testing procedures for fetomaternal hemorrhage detection, paroxysmal nocturnal hematuria screening, membrane defect anemia testing for hereditary spherocytosis, and nucleated RBC counting. Points of validation and quality control, and caveats of interpretation are also discussed.

This reaffirmed document has been reviewed and confirmed as suitable to remain published without revision to content, as of June 2016. The document’s next scheduled review is generally five years after the reaffirmation date.