CLSI I/LA26

Performance of Single Cell Immune Response Assays, 2nd Edition

Learn about methods for intracellular cytokine evaluation, major histocompatibility complex multimer quantitation, and enzyme-linked immunospot technology for the assessment of cellular proliferation with I/LA26. I/LA26 also provides basic aspects of specimen collection, transport, and preparation; results interpretation; and quality assurance and test validation approaches for single cell immune response assays.

This document is available in electronic format only.

More Details

Member price:

List Price:

PDF Add to Cart

Details

Chairholder: Maurice R.G. O’Gorman, MBA, MSc, PhD, D(ABMLI)

Date of Publication: November 27, 2013

Order Code PDF: CLSI ILA26A2E
ISBN Number: 1-56238-894-0

Order Code Print: print not available

Edition: Second

Pages: 106

CLSI I/LA26 Additional Details

If interested in ordering larger quantities of this document in print, please contact us here.

This reaffirmed document has been reviewed and confirmed as suitable to remain published without revision to content, as of September 2018. The document’s next scheduled review is generally five years after the reaffirmation date.

CLSI I/LA26 Abstract

Clinical and Laboratory Standards Institute document I/LA26-A2—Performance of Single Cell Immune Response Assays; Approved Guideline—Second Edition describes assays that measure antigen-specific cellular immune responses in the context of clinical trials and in the management of subjects with immune-mediated diseases. Immune therapeutic approaches are being applied in various fields of medicine, including infectious diseases, transplantation, autoimmune disease, cancer, and allergies. Assays are required to measure the cellular effects of such therapeutic approaches. This guideline focuses on the methods of intracellular cytokine evaluation, major histocompatibility complex multimer quantitation, enzyme-linked immunospot technology, and carboxyfluorescein succinimidyl ester tracking dye staining. The document covers basic aspects of specimen collection, transport, and preparation, in addition to QA and method validation approaches. Data acquisition, data analysis, and reporting aspects for these assays are also summarized.