CLSI M15
Laboratory Diagnosis of Blood-borne Parasitic Diseases
This CLSI document provides comprehensive guidelines for diagnosing blood-borne parasitic diseases. It includes detailed procedures for specimen collection through skin puncture and venipuncture, blood film preparation, and staining techniques. The document details the use of Giemsa stain and other reagents, including a special stain for microfilariae, and provides a thorough list of essential supplies. Additionally, it outlines optimal blood collection times for detecting Plasmodium (malaria), Babesia, Trypanosoma cruzi (Chagas' disease), African trypanosomiasis, and filariasis, helping laboratories improve detection and identification accuracy.
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{{FormatPrice(nonMemberPrice)}} List PriceCLSI document M15-A—Laboratory Diagnosis of Blood-borne Parasitic Diseases; Approved Guideline, presents instructions for preparation of thick and thin blood films, the appropriate use of stains, and methods to assist in the diagnosis of many parasitic diseases. Procedures for blood collection by skin puncture and venipuncture, techniques for preparing films for blood parasite examination, and steps for preparing Giemsa stain and other reagents, including a special stain for microfilariae, are provided. The optimum times for preparing blood films for five particular parasites—Plasmodium species (malaria), Babesia species, Trypanosoma cruzi (Chagas' disease), African trypanosomiasis, and filariasis—are identified and explained. A thorough list of blood film examination supplies is included. Basic guidelines and reference materials for the identification of blood parasites are given. Other than babesiosis, vector-transmitted, blood-borne parasitic diseases are not endemic to temperate climates. However, laboratories may be called upon to detect and identify an etiological agent in suspected infections. This document is, therefore, useful for the performance of such laboratory procedures.
This document is available in electronic format only.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.
CLSI document M15-A—Laboratory Diagnosis of Blood-borne Parasitic Diseases; Approved Guideline, presents instructions for preparation of thick and thin blood films, the appropriate use of stains, and methods to assist in the diagnosis of many parasitic diseases. Procedures for blood collection by skin puncture and venipuncture, techniques for preparing films for blood parasite examination, and steps for preparing Giemsa stain and other reagents, including a special stain for microfilariae, are provided. The optimum times for preparing blood films for five particular parasites—Plasmodium species (malaria), Babesia species, Trypanosoma cruzi (Chagas' disease), African trypanosomiasis, and filariasis—are identified and explained. A thorough list of blood film examination supplies is included. Basic guidelines and reference materials for the identification of blood parasites are given. Other than babesiosis, vector-transmitted, blood-borne parasitic diseases are not endemic to temperate climates. However, laboratories may be called upon to detect and identify an etiological agent in suspected infections. This document is, therefore, useful for the performance of such laboratory procedures.
This document is available in electronic format only.
This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid and because of its value to the laboratory community, it is being retained in CLSI’s library.
The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.