CLSI M15

Laboratory Diagnosis of Blood-borne Parasitic Diseases, 1st Edition

This document contains guidelines for specimen collection, blood film preparation, and staining procedures. Recommendations for optimum timing of specimen collection to assist laboratories in detecting, identifying and reporting certain parasites are also included.

This document is available in electronic format only.

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Chairholder: Lynne S. Garcia, MS, F(AAM)

Date of Publication: June 1, 2000

Order Code PDF: CLSI M15AE
ISBN Number: 1-56238-401-5

Order Code Print: print not available

Edition: First

Pages: 40

CLSI M15 Additional Details

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The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus guideline for use in satisfying a regulatory requirement.

This archived document is no longer being reviewed through the CLSI Consensus Document Development Process. However, this document is technically valid as of January 2017. Because of its value to the laboratory community, it is being retained in CLSI’s library.

CLSI M15 Abstract

CLSI document M15-A—Laboratory Diagnosis of Blood-borne Parasitic Diseases; Approved Guideline, presents instructions for preparation of thick and thin blood films, the appropriate use of stains, and methods to assist in the diagnosis of many parasitic diseases. Procedures for blood collection by skin puncture and venipuncture, techniques for preparing films for blood parasite examination, and steps for preparing Giemsa stain and other reagents, including a special stain for microfilariae, are provided. The optimum times for preparing blood films for five particular parasites—Plasmodium species (malaria), Babesia species, Trypanosoma cruzi (Chagas' disease), African trypanosomiasis, and filariasis—are identified and explained. A thorough list of blood film examination supplies is included. Basic guidelines and reference materials for the identification of blood parasites are given.