CLSI M44

Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts, 3rd Edition

Laboratorians can learn about an established methodology for disk diffusion testing of Candida spp., as well as recommendations for results interpretation and quality control testing.

Supplemental tables for M44 are published in CLSI document M27M44S.

This document is available in electronic format only.

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Chairholder: Gary W. Procop, MD, MS

Date of Publication: December 10, 2018

Order Code PDF: CLSI M44Ed3E
ISBN Number: 978-1-68440-031-7

Order Code Print: print not available

Edition: Third

Pages: 44

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A guideline for global application developed through the Clinical and Laboratory Standards Institute consensus process.

CLSI M44 Abstract

Clinical and Laboratory Standards Institute guideline M44—Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts provides an approved method for determining zone diameter breakpoints for select Candida spp. with antifungal agents after 24 hours incubation, as well as quality control parameters for the same agents. This guideline fulfills the need for an alternative to the broth microdilution testing procedure (see CLSI document M271) and describes a simple, rapid, and cost-effective approach for determining fungal organisms’ susceptibility to various classes of antifungal agents. It also makes antifungal susceptibility testing more readily available to the medical microbiology laboratory and encourages the development of disk diffusion testing for newly discovered antifungal agents.