eLearning
Microbiology

CLSI M100 Course

Using CLSI M100 | Performance Standards for Antimicrobial Susceptibility Testing

Improve your laboratory's antimicrobial susceptibility testing (AST) by using this self-paced online learning program. This interactive program will teach you how to navigate the many tables found in the 35th Edition of standard document CLSI M100 | Performance Standards for Antimicrobial Susceptibility Testing, 35th Edition (2025).

During this online learning course, you will learn: 

  • More about CLSI and its role in AST. 
  • How to locate specific tables in CLSI M100 that can guide AST testing and reporting decisions, such as: 
    • Selecting antimicrobial agents for testing and reporting. 
    • Interpreting zone diameter and minimal inhibitory concentration (or MIC) measurements. 
    • Choosing organisms for quality control of disk diffusion and MIC tests.

This course is offered free of charge as a public health service given the importance of AST in combatting the spread of antimicrobial resistance (AMR). Other AST resources can be found here in the CLSI shop and you can learn more about CLSI's expertise and resources here on the AST page.

March 25, 2025
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Scope

Learning Objectives

  • Describe CLSI and its role in antimicrobial susceptibility testing.
  • Locate specific tables in CLSI M100 that can guide antimicrobial susceptibility testing and reporting decisions to include, selecting antimicrobial agents for testing and reporting, interpreting zone diameter and MIC measurements, and choosing organisms for quality control of disk diffusion and MIC tests. 

Basic: Entry level. No prior knowledge of subject necessary.

CLSI is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program.

Authors
Janet A. Hindler, MCLS, MT(ASCP), F(AAM)
Shelley Campeau, PhD, D(ABMM)
Audrey N. Schuetz, MD, MPH, D(ABMM)
Supporting Resources
CLSI M100Plus
M100 Plus
Subscription
Free
Scope

Learning Objectives

  • Describe CLSI and its role in antimicrobial susceptibility testing.
  • Locate specific tables in CLSI M100 that can guide antimicrobial susceptibility testing and reporting decisions to include, selecting antimicrobial agents for testing and reporting, interpreting zone diameter and MIC measurements, and choosing organisms for quality control of disk diffusion and MIC tests. 

Basic: Entry level. No prior knowledge of subject necessary.

CLSI is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program.

Authors
Janet A. Hindler, MCLS, MT(ASCP), F(AAM)
Shelley Campeau, PhD, D(ABMM)
Audrey N. Schuetz, MD, MPH, D(ABMM)
Supporting Resources
CLSI M100Plus
M100 Plus
Subscription
Free