Webinar
Clinical Chemistry and Toxicology
CLSI C64 Webinar
Quantitative Measurement of Proteins and Peptides by Mass Spectrometry Webinar
By the end of this webinar, you will have learned about:
- The role that protein quantitation by mass spectrometry plays in the clinical laboratory.
- The uniquely challenging aspects of protein quantitation.
- How C64 provides a framework for development of protein assays that perform adequately for clinical deployment.
August 26, 2021
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Syllabus
Clinical and Laboratory Standards Institute guideline C64—Quantitative Measurement of Proteins and Peptides by Mass Spectrometry provides a framework for developing clinical protein and peptide assays from conception to validation. This guideline is intended for those who have experience with traditional small-molecule liquid chromatography–mass spectrometry (LC-MS) but not with protein and peptide analysis. Although closely related to traditional small-molecule analysis by LC-MS, protein and peptide analysis involves unique challenges and necessitates complex workflows, which are covered in detail. To enhance translation of assays to clinical use, this guideline focuses on method development aligned with clinically appropriate analytical validation.
Scope
Learning Objectives
- Describe the role that protein and peptide quantitation by mass spectrometry plays in the clinical laboratory.
- Provide insight into the uniquely challenging aspects of protein and peptide quantitation by mass spectrometry.
- Share how C64 provides a framework for the development of protein and peptide assays that have performance characteristics suitable for clinical deployment.
Syllabus
Clinical and Laboratory Standards Institute guideline C64—Quantitative Measurement of Proteins and Peptides by Mass Spectrometry provides a framework for developing clinical protein and peptide assays from conception to validation. This guideline is intended for those who have experience with traditional small-molecule liquid chromatography–mass spectrometry (LC-MS) but not with protein and peptide analysis. Although closely related to traditional small-molecule analysis by LC-MS, protein and peptide analysis involves unique challenges and necessitates complex workflows, which are covered in detail. To enhance translation of assays to clinical use, this guideline focuses on method development aligned with clinically appropriate analytical validation.
Scope
Learning Objectives
- Describe the role that protein and peptide quantitation by mass spectrometry plays in the clinical laboratory.
- Provide insight into the uniquely challenging aspects of protein and peptide quantitation by mass spectrometry.
- Share how C64 provides a framework for the development of protein and peptide assays that have performance characteristics suitable for clinical deployment.