Nucleic Acid Sequencing Methods in Diagnostic Laboratory Medicine, 2nd Edition

This document addresses diagnostic sequencing using both automated capillary-based sequencers and massively parallel sequencing instruments. Topics include specimen collection and handling; isolation and extraction of nucleic acid; template preparation; sequence generation, alignment, and assembly; validation and verification; ongoing quality assurance; and reporting results. 

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Publication Details

Chairholder: Thomas J. Lenk, PhD and Ira M. Lubin, PhD, FACMG

Date of Publication: February 28, 2014

ISBN Number: 1-56238-953-X

Order Code PDF: MM09A2E

Order Code Print: MM09A2

Edition: Second

Pages: 140

MM09 Additional Details

The U.S. Food and Drug Administration (FDA) has evaluated and recognized this approved-level consensus standard for use in satisfying a regulatory requirement.