CLSI VET03

Methods for Antimicrobial Broth Dilution and Disk Diffusion Susceptibility Testing of Bacteria Isolated From Aquatic Animals, 2nd Edition

This guideline provides the most up-to-date techniques for the determination of minimal inhibitory concentrations and zones of inhibition of aquatic bacteria and criteria for data interpretation and quality control testing.

Supplemental tables for VET03 are published in CLSI document VET04.

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Chairholder: Brian V. Lubbers, DVM, PhD, DACVCP

Date of Publication: May 20, 2020

Order Code PDF: CLSI VET03Ed2E
ISBN Number: 978-1-68440-074-4

Order Code Print: CLSI VET03Ed2
ISBN Number: 978-1-68440-073-7

Edition: Second

Pages: 122

CLSI VET03 Additional Details

A guideline for global application developed through the Clinical and Laboratory Standards Institute consensus process.

CLSI VET03 Abstract

Clinical and Laboratory Standards Institute guideline VET03—Methods for Antimicrobial Broth Dilution and Disk Diffusion Susceptibility Testing of Bacteria Isolated From Aquatic Animals describes broth dilution and disk diffusion, and it includes a series of procedures to standardize the way the tests are performed on Groups 1 and 3 aquatic bacteria. Group 1 nonfastidious bacteria grow readily in cation-adjusted Mueller-Hinton broth (CAMHB) and on Mueller-Hinton agar and are readily cultured at temperatures of 22°C ± 2°C and 28°C ± 2°C. Group 3 nonfastidious gliding bacteria grow in diluted CAMHB and are readily cultured at temperatures of 18°C or 28°C, depending on the species.